Peptide Reconstitution Calculator

Enter the vial size, how much bacteriostatic water you added, and your target dose. Get the concentration, the volume to draw, and — the number most people actually need — the units to fill on a U-100 insulin syringe.

Research use only. This is a dilution and volume calculator — it computes the arithmetic you enter. It is not dosing advice and makes no medical, safety, or efficacy claims. It does not tell you what to dose, only the math for the numbers you provide.

Quick presets — prefill a typical vial size, then adjust

Inputs

mg
mL
mcg = micrograms · mg = milligrams (1 mg = 1000 mcg)

Results

Concentrationpeptide per mL of solution 5.00mg/mL
Volume to drawper dose 0.100mL
Total doses per vialat this dose 20doses
10
units on a U-100 insulin syringe
draw to the 10-unit mark

How peptide reconstitution math works

Reconstitution is just dilution. When you add bacteriostatic water to a lyophilized (freeze-dried) peptide vial, the peptide dissolves into that volume of liquid. The concentration is the peptide amount divided by the water you added:

concentration (mg/mL) = vial amount (mg) ÷ bac water (mL)

To pull a specific dose, you divide the dose by that concentration to get the volume to draw. Because a U-100 insulin syringe is marked so that 100 units = 1 mL, multiplying the draw volume by 100 gives the unit mark to fill to — the number most people read off the barrel:

volume (mL) = dose (mg) ÷ concentration (mg/mL)
units (U-100) = volume (mL) × 100

Worked example

A 10 mg vial reconstituted with 2 mL of bac water gives 10 ÷ 2 = 5 mg/mL. For a 0.5 mg (500 mcg) dose: 0.5 ÷ 5 = 0.1 mL, which is 0.1 × 100 = 10 units on a U-100 syringe.

Why bac water volume is a choice, not a rule

The water you add doesn't change the total amount of peptide in the vial — only how concentrated each draw is. More water means a larger, easier-to-measure draw at a lower concentration; less water means a smaller draw. Pick a volume that lands your typical dose on a readable mark. This tool only does the arithmetic; it does not recommend a dose or a dilution.