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semaglutide glp 1 agonist in vitro n preclinical 2026-05-04 PubMed

Optimizing Oral Semaglutide Formulation with Absorption Enhancer Using Quality by Design

Quality by Design-Based Formulation Development of an Oral Semaglutide Tablet.

Background

Oral delivery of semaglutide, a glucagon-like peptide-1 (GLP-1) receptor agonist used for type 2 diabetes and obesity, faces significant challenges due to its peptide nature, leading to poor bioavailability. Sodium caprate (C10) is often used as an intestinal absorption enhancer to overcome this. This study aimed to scientifically optimize an oral semaglutide tablet formulation incorporating C10 using a Quality by Design (QbD) approach.

Study Design

Population
This in-vitro study focused on optimizing an oral semaglutide tablet formulation, using Caco-2 cell monolayers as a model for intestinal absorption.
Intervention
The intervention involved an optimized oral semaglutide tablet formulation incorporating 20 mg of sodium caprate (C10) as an absorption enhancer.
Comparator
The optimized formulation was compared to non-optimized preliminary formulations and formulations without C10.
Outcome
The primary outcomes measured were the dissolution profile (95% drug release within 30 minutes), stability (less than 5% degradation over 6 months), and in vitro permeability across Caco-2 cell monolayers (1.8-fold increase in transport).

Results

The QbD approach successfully identified an optimal formulation for the oral semaglutide tablet. The optimized formulation demonstrated superior dissolution profiles, achieving 95% drug release within 30 minutes, representing a 2.3-fold increase compared to non-optimized preliminary formulations. Stability studies under accelerated conditions (e.g., 40°C/75% RH) showed less than 5% degradation of semaglutide over 6 months, indicating robust shelf-life potential. The inclusion of sodium caprate (C10) at an optimized concentration of 20 mg per tablet significantly enhanced in vitro permeability across Caco-2 cell monolayers (a common model for intestinal absorption), resulting in a 1.8-fold increase in semaglutide transport compared to formulations without C10. > The optimized oral semaglutide tablet formulation, developed using QbD principles, achieved significantly improved dissolution and permeability, alongside excellent stability, addressing key challenges in oral peptide delivery.

Why It Matters

This study provides a robust, scientifically-driven methodology for developing complex oral peptide formulations like semaglutide, potentially leading to more effective and patient-friendly treatment options. The enhanced dissolution and stability of the optimized tablet could improve bioavailability and reduce dosing variability, thereby increasing patient adherence for chronic conditions like type 2 diabetes and obesity. Successful translation of these findings into human clinical trials could pave the way for a new generation of highly effective and convenient oral GLP-1 receptor agonists. The next steps involve in vivo animal studies to confirm bioavailability and efficacy, followed by Phase I and II human clinical trials.


semaglutide glp 1 agonist glp-1r dose mentioned
Source: pubmed:42076092 · Ingested 2026-05-04 · Digest: gemini-2.5-flash