Dysregulated Hippo-related ceRNA network investigated for links to diminished ovarian reserve and IVF outcomes

Diminished Ovarian Reserve (DOR) is a significant contributor to female infertility, characterized by poor ovarian response and reduced pregnancy rates during In Vitro Fertilization (IVF). The precise molecular mechanisms impairing follicular development in DOR remain largely undefined. Emerging evidence highlights the crucial roles of non-coding RNAs and components of the Hippo signaling pathway in regulating granulosa cell proliferation, apoptosis, and overall follicular development, suggesting potential targets for understanding and addressing DOR.

This prospective observational cohort study aims to investigate the expression of specific molecular markers in follicular fluid-derived cells. Researchers will compare women with DOR undergoing IVF with women exhibiting normal ovarian reserve. The markers under investigation include the long non-coding RNA (lncRNA) Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), microRNA (miRNA)-181a-5p, and key Hippo pathway components: Yes-Associated Protein 1 (YAP1) and Connective Tissue Growth Factor (CTGF), alongside Insulin-Like Growth Factor 1 (IGF1). The study will evaluate relationships among these molecular markers and IVF outcomes, including oocyte quality, number of retrieved oocytes, and embryo developmental potential.